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Isolation and quantitation of BHV-1, vBHV-1lox (wt), and vBHV-1gEAm453 viruses present in nasal (A) and ocular (B) swabs of calves during the acute, latent, and postreactivation stages of infection. Virus was isolated at the indicated intervals. Virus quantitation was performed by plaque assay on MDBK cells, and data represent averages and standard deviations for each group.
