FIG. 5.

Biochemical assessment of the effects of the mutations introduced in eIF4E on eIF4G binding. The results of an ELISA representative of three independent experiments are shown. Wells were precoated with 1 μg of eIF4E protein and incubated with either 2 μg of wheat eIF4G (hatched bars) or no protein (black bars). (A) Titration of the coated protein was carried out using polyclonal antibodies against eIF4E. (B) Binding complexes were detected using polyclonal antibodies against eIF4G. The absorbance values at 405 nm of four replicates and standard deviations are given.