TABLE 1.
Summary of the properties of the LMV-4E mutants in susceptible and resistant lettuce
| Class of mutation | eIF4E mutation | Delay in symptom emergencea
|
Reduced virus accumulationb
|
||||
|---|---|---|---|---|---|---|---|
| Salinas (mo10) | Salinas 88 (mo12) | Mantilia (mo11) | Salinas (mo12) | Salinas 88 (mo10) | Mantilia (mo11) | ||
| eIF4G binding | E91A | − | − | − | − | − | > |
| W94A | − | − | − | − | − | − | |
| G156A | − | − | − | − | * | − | |
| E157A | − | − | − | − | − | − | |
| Cap binding | W64A | − | * | * | − | *** | ** |
| W77L | − | − | ** | * | − | *** | |
| W123A | − | − | − | − | − | − | |
| R173A | − | *** (WA)c | ** | − | *** (WA) | *** | |
| W182A | − | ** (WA) | ** | − | *** (WA) | *** | |
| S223L | − | − | − | > | > | − | |
| Outer surface | F65A | − | * | *** | − | *** | − |
| R82L | − | − | − | − | > | − | |
| Y113A | − | − | − | − | − | − | |
| A174P | − | − | − | − | > | − | |
a
−, no difference with LMV-4E0; *, delay of more than 3 days after emergence of symptoms induced by LMV-4E0; **, more than 6 days; ***, more than 14 days.
b
−, no significant difference in accumulation compared to LMV-4E0; *, reduction of more than 15%; **, more than 40%; ***, more than 70%; >, accumulation increased compared to that of LMV-4E0.
c
WA (when applied) means that in only one experiment of three were LMV accumulation and symptoms observed in the resistant lettuce, although in susceptible plants inoculated with the same LMV inoculum, infection occurred in three experiments of three.