TABLE 2.

Dissociation constants of the different forms of eIF4E with their ligands

eIF4E form	Kd (106 mol/liter)
	m7GTP	VPg	pep4G
eIF4E0	0.25 ± 0.01	0.27 ± 0.03	0.11 ± 0.01
eIF4G-binding mutants
E91A	NDa	ND	0.23 ± 0.01
G156A	ND	ND	0.30 ± 0.01
E157A	ND	ND	0.43 ± 0.02
Outer-surface mutants
R82L	0.41 ± 0.03	1.28 ± 0.07	ND
F65A	0.75 ± 0.05	2.41 ± 0.14	ND
A174P	1.56 ± 0.17	14.1 ± 2.3	ND

^aND, not determined.