TABLE 3.
Summary of the biochemical and biological properties of Ls-eIF4E mutants
| Class of mutation | eIF4E form | In vitro cap bindinga,b | In vitro eIF4G bindingb | Virus rescuec | Yeast rescued |
|---|---|---|---|---|---|
| mo10 allele | eIF4E0 | + | + | + | + |
| mo11 allele | eIF4E1 | ± | NT | − | + |
| mo12 allele | eIF4E2 | ± | NT | − | + |
| eIF4G binding | E91A | + | ± | + | + |
| W94A | + | ± | + | + | |
| G156A | + | ± | + | + | |
| E157A | + | ± | + | −* | |
| Cap binding | W64A | − | NT | − | − |
| W77L | ± | NT | − | − | |
| W123A | − | NT | + | − | |
| R173A | + | NT | − | − | |
| W182A | − | NT | − | − | |
| S223L | + | NT | + | + | |
| Outer surface | F65A | ± | NT | − | − |
| R82L | + | NT | + | + | |
| Y113A | + | NT | + | + | |
| A174P | ± | NT | + | −* |
a
Estimated by cap affinity chromatography.
b
+, present; −, absent; ±, binding is affected, but not fully abolished; NT, not tested.
c
+, the eIF4E form is able to complement LMV infection in resistant plants; −, no complementation occurs.
d
10× serial dilutions of yeast strain J055 transformed with plasmids p426GPD-4E were plated on glucose selective medium. +, 100× or 1,000× dilutions grew on glucose-containing medium; -, these dilutions did not grow (behavior was identical to that of the negative control, cells transformed with the plasmid p426GPD); *, in only one experiment of 4 did cells grow for a 10× dilution, but none grew for the 100× and 1,000× serial dilutions.