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. 2008 May 28;82(15):7666–7676. doi: 10.1128/JVI.02274-07

FIG. 5.

FIG. 5.

Differential lysis of parental and replicon-expressing cells by NK92 and NK92/KIR2DL1-GFP cell lines. NK92/KIR2DL1-GFP cell cytotoxicity is impaired against K562 cells expressing the DV replicon. K562 and K562.ΔCprME-PAC2A cells were used as targets in a standard 5-h 35S release assay. (A) Representative assay. (Top) K562; (bottom) K562.ΔCprME-PAC2A. (B) The cytotoxic activity of NK92 cells was compared to the cytotoxic activity of NK92/KIR2DL1-GFP cells, and activity was normalized by dividing target-specific lysis activity of NK92/KIR2DL1-GFP cells by that of NK92 cells. Values are means of two different experiments. *, P < 0.02; **, P < 0.0002 (as analyzed by single-factor analysis of variance). (C) Reduced cytotoxic activity of NK92 cell line against THP-1 cells expressing the DV replicon compared to that of the parental THP-1 cells. THP-1 and THP-1.ΔCprME-PAC2A cells were labeled with 35S and then incubated with NK92 at various E:T ratios for 5 h. The value for each time point is the mean of the percent specific lysis of four separate wells.