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. 2008 May 28;82(15):7666–7676. doi: 10.1128/JVI.02274-07

FIG. 6.

FIG. 6.

Lysis of K562 and K562.ΔCprME-PAC2A cells by the primary NK line and NK clones. (A) Reduced cytotoxic activity of the primary NK line against K562 cells expressing the DV replicon. Primary NK cells prepared as described in Materials and Methods were incubated for 5 hours with labeled K562 and K562.ΔCprME-PAC2A cells. Results are from one representative experiment out of two performed. The value for each time point is the mean of the percent specific lysis of four separate wells. *, P < 0.04, as analyzed by single-factor analysis of variance. (B and C) NK clone lysis against K562 and K562.ΔCprME-PAC2A cells. Clones were stained for the presence of KIR2DL1 and KIR2DL2 receptors using anti-KIR2DL1 and anti-KIR2DL2 MAbs. (B) Lysis of K562 and K562.ΔCprME-PAC2A cells by two representative NK clones expressing only KIR2DL2 (AB3) or both KIR2DL1 and KIR2DL2 (AB2). (C) Lysis of K562 and K562.ΔCprME-PAC2A cells by two representative NK clones (CD3 and EF9) that don't express either KIR2DL1 or KIR2DL2. Results for all the lysis assays presented are from one representative experiment out of two performed. The value for each time point is the mean of the percent specific lysis of four separate wells.