FIG. 1.

Murine B cells do not support MHV68 lytic replication. (A) Murine B-cell lines A20 and WEHI-231, myeloma cell line NSO, and NIH 3T12 fibroblasts were infected with MHV68 at an MOI of 10 PFU/cell. Cells were harvested at the indicated times postinfection, and viral titers were determined by plaque assay. Results are means of triplicate samples. Error bars represent standard deviations. (B) RT-PCR analyses of spliced orf50 (Rta) and orf73 (mLANA) transcripts 24 h postinfection. Spliced orf50 transcripts (indicated by arrows) are associated with and required for lytic replication. Spliced orf73 transcript are present during lytic and latent phases of infection.