FIG. 1.

Samples and sorting strategy for TCR repertoire analysis. (a) Sorted KP9-specific CD8 T cells (indicated by dots) were derived from two animals receiving VV/FPV vaccines (total, six samples; left panel) and two animals receiving DNA/FPV vaccines (total, four samples; right panel). Vaccination time points are indicated by upward-pointing arrows, along with the vaccine modalities administered. Viral challenge is indicated by a downward-pointing arrow. (b) Gating strategy used to sort KP9-specific CD8 T cells. Thawed pigtail macaque PBMC samples were stained with CD3, CD8, KP9 tetramer, CD45RA, CD28, and ViVid viability dye. Viable single CD3⁺ tetramer⁺ lymphocytes, and singlets were sorted into RNAlater with a modified BD FACSAria under biosafety level 3 conditions and used for TCR repertoire analysis.