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. 2008 May 28;82(15):7357–7368. doi: 10.1128/JVI.00607-08

FIG. 6.

FIG. 6.

Recognition of KP9 mutants. Peptides containing sequential alanine mutations across the KP9 epitope were studied in IFN-γ intracellular cytokine assays of whole blood from Mane-A*10+ animals with KP9-specific CD8 T-cell responses. (a) Alanine scan titration of blood from VV/FPV-immunized animal 5827. (b) Comparative reactivity of each alanine mutant with wild-type (WT) KP9 peptide at 100 ng/ml (from the titration shown in panel a). (c) Additional alanine scan titration, including the common K165R and P172S mutations, of a separate blood sample. (d) Comparative reactivity of each alanine mutant with the WT KP9 peptide at 100 ng/ml (from the titration shown in panel c). A1 = AKFGAEVVP, A2 = KAFGAEVVP, A3 = KKAGAEVVP, A4 = KKFAAEVVP, K5 = KKFGKEVVP, A6 = KKFGAAVVP, A7 = KKFGAEAVP, A8 = KKFGAEVAP, A9 = KKFGAEVVA, K165R = KRFGAEVVP, and P172S = KKFGAEVVS (mutations are in bold).