TABLE 1.
TCR repertoire analysis
| Animal | Vaccine, challenge virus (reference) | Sample | Time point (wk) | % Tetramera | No. of cellsb | TCR clone no.c |
|---|---|---|---|---|---|---|
| 5821 | VV/FPV-4-1BBL, SIVmac251 (32) | Prechallenge | 5 | 0.47 | 237 | 81 |
| Postchallenge acute | 19.5 | 3.17 | 10,000 | 83 | ||
| Postchallenge chronic | 36 | 1.4 | 2,500 | 50 | ||
| 5827 | VV/FPV-4-1BBL, SIVmac251 (32) | Prechallenge | 5 | 0.15 | 800 | 65 |
| Postchallenge acute | 19.5 | 4.15 | 5,000 | 84 | ||
| Postchallenge chronic | 36 | 1.7 | 5,000 | 70 | ||
| 5616 | DNA/FPV, SHIVmn229 (9) | Prechallenge | 12 | 0.14 | 530 | 75 |
| Postchallenge acute | 25 | 24.19 | 5,000 | 79 | ||
| 6276 | DNA/FPV, SHIVmn229 (9) | Prechallenge | 12 | 0.14 | 300 | 93 |
| Postchallenge acute | 25 | 26.91 | 10,000 | 75 | ||
| M18 | Live-attenuated SIV (20) | Postchallenge acute | 7 | 0.21 | 700 | 63 |
a
The tetramer responses listed were measured in fresh whole blood at the original trial time points, except for the animal M18 sample response and the animal 5821 and 5827 postchallenge chronic sample responses, which were measured in thawed PBMC samples.
b
Shown is the number of cells obtained by sorting and subsequently used for RNA extraction and TCR repertoire analysis.
c
Ninety-six colonies were screened for each sample. The TCR clone number is the number of colonies that yielded viable TCR sequence and were therefore included in the analysis.