FIG. 1.

ERK1/2 is involved in HAstV replication. (A) Caco-2 cells were treated with media alone, HAstV-1 (MOI of 20), or an equal amount of UV-inactivated HAstV-1 and cell lysates collected from 15 min pi to 8 hpi. As positive and negative controls, Caco-2 cells were preincubated with U0126 (10 μM) or vehicle control for 1 h and subsequently stimulated with EGF (10 nM). At the indicated times, cell lysates were separated by SDS-PAGE and the amounts of phosphorylated and total ERK1/2 evaluated by Western blotting. (B) Caco-2 cells were preincubated with increasing concentrations of U0126 or PD98059 or with vehicle control and then mock or HAstV-1 (MOI of 5) infected in the presence of drug. Viral production was measured by ELISA at 24 hpi. Data are representative of the results of at least two experiments. *, P < 0.05; error bars represent standard deviations.