FIG. 2.

ERK1/2 requirement is independent of HAstV serotype or cell type. (A) Caco-2 cells were preincubated with U0126 (10 μM; black bars) or DMSO (0.1%; white bars) and infected with HAstV-1, HAstV-2, or HAstV-8 (MOI of 5). Viral production at 24 hpi was monitored by ELISA. (B) Caco-2, LLC-MK2, Vero, and 293 cells were pretreated with U0126 (10 μM; white bars) or DMSO (0.1%; black bars) and infected with HAstV-2 (MOI of 5) in the presence of drug. Viral production was measured by ELISA at 24 hpi. ELISAs were performed in triplicate; data are representative of the results of at least three independent experiments. *, P < 0.05; error bars represent standard deviations.