FIG. 4.

ERK inhibition has a modest effect on cell death during HAstV infection. Caco-2 cells were preincubated in the presence of DMSO (0.1%), U0126 (10 μM), or PD9805 (50 μM). Cells were then mock or HAstV-1 (MOI of 5) infected in the presence of drug; viability was measured by an MTS assay of Caco-2 cells at 12 or 24 hpi (A) or of 293 cells at 12 hpi (B). Mock infection, black bars; HAstV-1 infection, white bars; D, DMSO; U, U0126; P, PD98059. *, P < 0.05 compared to drug control results; error bars represent standard deviations.