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KΔUL36GFP was present in a membrane-associated float-up fraction. Vero cells were infected with KΔUL36GFP at an MOI of 50. At 12 h postinfection cells were harvested and then PNS was prepared and loaded at the bottom of a sucrose gradient. A membrane-associated buoyant fraction was isolated and flowed into a microchamber containing rhodamine-labeled microtubules. HSV membrane-associated particles are shown in green and microtubules are in red. Bar, 10 μm.
