FIG. 1.

HIV-1 vector constructs with cPPT and RT mutations. (A) The transfer pHR'CMV-GFP transfer vector (27) expressing eGFP from the cytomegalovirus (CMV) promoter was modified to contain a 15-bp cPPT sequence (5′-AAAAGAAAAGGGGGG-3′) at the ClaI site at the beginning of the CMV promoter sequence. In this construct, 3′ PPT and cPPT primers synthesize 2,180- and 2,030-nucleotide-long (+)-strand DNAs. (B) The Q151N and V148I mutations were created in the pCMVΔR8.2 HIV-1 packaging plasmid by site-directed mutagenesis. These vectors were pseudotyped by use of the vesicular stomatitis virus G envelope.