FIG. 2.

Transduction of WT and RT mutant HIV-1 vectors in primary HLFs. HLFs cultured with 10% serum (3 × 10⁵) were transduced with 2.8 × 10⁵-pg p24 of WT or Q151N or V148I RT mutant vector with the inset mean fluorescence intensity values (A), and the percentages of the transduced cells were determined by FACS for eGFP expression at 48 h posttransduction (B). The experiments were performed in triplicate and values are presented with standard deviation of the mean.