FIG. 4.
Effect of cPPT and dN treatment on the transduction of WT and Q151N HIV-1 vectors in HLFs cultured in the presence or absence of 10% serum. HLFs cultured with 10% (dividing) or with 0.1% (nondividing) serum for 3 days were transduced with the (−) or (+) cPPT WT or Q151N vector (2.8 × 105 pg p24), and the transduction was determined by FACS for GFP expression at 48 h posttransduction. The 2.5 mM dN treatment was performed at 5 h before transduction as previously described (12). Data are presented with standard deviation of the mean.