FIG. 8.

Inhibition of transcription in cells infected with wild-type or mutant viruses. (A) BHK-15 cells were infected with SINV, R615A, or SIN/G mutants at an MOI of 10 and maintained at 37°C. At 6 h postinfection cellular RNAs were labeled with inorganic ³²P for 3 h, and the RNA was extracted and analyzed by gel electrophoresis under the conditions described in Materials and Methods. The positions of 28S and 18S rRNA bands are indicated. G and Sg indicate the viral genomic and subgenomic RNAs, respectively. Asterisks indicate the rRNA precursors. (B) The synthesis of cellular RNA in infected cells was analyzed by measuring the radioactivity counts in the 18S rRNA band.