TABLE 1.
Plaque phenotypes at permissive and nonpermissive temperatures of growth
| VEEV nsP2 residue | SINV nsP2 mutationa | Plaque phenotypeb
|
|
|---|---|---|---|
| 30°C | 37°C | ||
| R604 | R615A | Medium | NP |
| K646 | K658A | Large | Large |
| M647 | R659A | Large | Large |
| K673 | R686A | Large | Large |
| K686 | R699A | Medium | NP |
| I720 | K733A | Medium | Small |
| R727 | R740A | Large | Medium |
| R738 | R751A | Small | Small |
| Q739 | K752A | NP | NP |
| K747 | R760A | Large | Medium |
| K767? | R781A | Large | Large |
| R769 | R783A | Large | Large |
| SINV (wild-type) | Large | Large | |
a
The SINV residue analogous to the respective VEEV residue is given in each case.
b
Plaque sizes are defined as follows: large, 2.0 to 2.5 mm; medium, 1.0 to 2.0 mm; small, ≤0.5 mm. NP, no plaques after 24 h at 37°C or 48 h at 30°C.