TABLE 3.

Phenotypic characteristics of nsP2 interdomain loop mutants^a

Mutation	Plaque phenotype	nsP2 synthesis in vivob
SINV	Large	+
N609A	NP	−
L610A	Small	I
V611A	Large	+
P612A	Large	+
V613A	Large	+
N614A*	NP (ts)	+
R615A*	NP (ts)	+
N616A	Large	+
L617A	Large	+
P618A	Large	+
H619A	Medium	+
A620R	Large	+
L621A	NP	−
V622A	Large	+

^aPlaque phenotype and nsP2 synthesis represent results obtained at 37°C. Plaque phenotype analysis was also performed at 30°C for all mutants that did not form plaques at 37°C to identify a temperature-sensitive (ts) lethal phenotype (*). NP, no plaques.

^bI, polyprotein intermediates observed in addition to full-length nsP2.