FIG. 2.

Size exclusion chromatography of P49 and P35. (A) P49 profile. Purified E. coli-produced P49-His₆ (top) or freeze-thaw extracts (bottom) of SF21 cells transfected with an ie-1 promoter expression plasmid encoding P49 were subjected to size exclusion chromatography and immunoblot analysis with anti (α)-P49. Chromatographic size standards are indicated across the top with fraction numbers. Electrophoretic molecular mass markers are indicated on the left. (B) P35 profile. Purified E. coli-produced P35-His₆ (top) or freeze-thaw extracts (bottom) of SF21 cells transfected with a plasmid encoding P35 were analyzed with anti-P35 as described for panel A. Arrowheads denote the peak fractions of P49 and P35.