FIG. 2.

Induction of differentiation markers in human colon adenocarcinoma cells after butyrate treatment. Cells were incubated in the presence or absence (control) of 4 mM butyrate for 48 h; afterwards, annexin A1 protein expression was analyzed by Western blotting (A), alkaline phosphatase activity by measuring the hydrolysis of p-nitrophenyl phosphate (B), and DPP-IV surface expression by flow cytometry (C). Blots in panel A and histograms in panel C are representative of four independent experiments; data in panel B correspond to mean values (± SD) for three independent experiments with quadruplicate samples. C, control; T, treated cells.