FIG. 3.

Basal annexin A1 promoter activities and effects of butyrate and TSA treatments. (A) Scheme of the different annexin A1 promoter fragments employed in luciferase assays showing the binding sites for transcription factors relative to the transcription start position (+1). GRE, glucocorticoid response element. (B) Promoter activities of the A1H-1, Ax1.3, A1H-5, and A1H-6 constructs in BCS-TC2 and BCS-TC2.BR2 cells measured with dual luciferase assays in exponentially growing cells. (C) Effects of treatment with butyrate or TSA for 24 h on the luciferase activities of different fragments of the annexin A1 promoter in BCS-TC2 and BCS-TC2.BR2 cells. Luciferase activities are expressed as relative induction levels, with the corresponding untreated cells used as a control. Data correspond to mean values (± SD) for three independent experiments performed with triplicate samples.