FIG. 5.

NF-YA and p53 protein expression and interaction. (A) Western blot analyses for NF-YA and p53 were carried out using total or nuclear cell extracts from BCS-TC2 and BCS-TC2.BR2 cells treated with butyrate or TSA for 24 h. Blots using anti-lamin B1 and anti-vinculin were used as loading controls in nuclear and total extracts, respectively. (B) Immunoprecipitated (IP) material with antibodies against NF-YA in nuclear extracts from BCS-TC2 or BCS-TC2.BR2 cells was probed for the presence of NF-YA and p53 by Western blot analysis with the corresponding antibodies. An immunoprecipitation-negative control without specific antibodies is included (*). Representative blots from four independent experiments are shown.