FIG. 6.

Influence of p38 MAPK activity in annexin A1 protein induction. (A) p38 MAPK activation after butyrate treatment was assessed by analysis of its phosphorylation in extracts obtained from control and butyrate-treated BCS-TC2 and BCS-TC2.BR2 cells. Total levels of p38 MAPK are shown as a control. (B and C) Involvement of p38 MAPK in annexin A1 protein expression induction by butyrate was assessed after treatment of the cells with the specific inhibitor SB203580 either in the absence or in the presence of butyrate. (B) Representative Western blot using 25 μM SB203580 in the presence or absence of butyrate treatment. (C) Quantification of the effects of different concentrations of SB203580 on annexin A1 expression referred to controls in the absence of butyrate and in the presence of the indicated concentration of p38 MAPK inhibitor. Data in panel C correspond to mean values (± SD) obtained from the densitometric analysis of blots from three independent experiments.