FIG. 6.
ddc1-T602A and dpb11-1 mutations are epistatic for UV sensitivity and effect on Rad53 phosphorylation. (A) Strain YFP63 (WT), YFP64 (ddc1-T602A), YFP65 (dpb11-1), and YFP66 (dpb11-1 ddc1-T602A) cells were arrested with nocodazole and UV irradiated. Rad53 phosphorylation was assayed 30 min after treatment. (B) Strains in panel A, YFP27 (dot1Δ), YFP142 (dot1Δ dpb11-1), YFP37 (dot1Δ ddc1-T602A), and YFP144 (dot1Δ ddc1-T602A dpb11-1) were grown overnight to stationary phase, and then 10-fold dilutions were spotted onto appropriate plates and either mock treated or UV irradiated with the indicated dosages. Images were taken after 3 days to measure cell survival.