FIG. 9.
Dpb11 is required for the full activation of Mec1. (A) Protein extracts from strain YMAG78/4B (dpb11td) were prepared under different conditions to assess the presence of the Dbp11-degron protein. Cells were cultured at 28°C in YP plus raffinose (Mock Unind.) and then shifted to the degradation medium at 37°C (Mock Induced), UV irradiated, and shifted back to 25°C (T0, sample taken immediately; T30, sample taken 30 min later). The presence of Dpb11-degron was assessed by using anti-HA antibodies. (B) The experiment was repeated under the same conditions in parallel with strains YMAG82/15a (DPB11) and YMAG78/4B (dpb11td). Ddc2 hyperphosphorylation was monitored by SDS-PAGE and Western blotting. CTRL, control.