TABLE 1.
Strains used in this study
| Strain | Relevant genotype | Source |
|---|---|---|
| K699 | MATaade2-1 trp1-1 leu2-3,112 his3-11,15 ura3 can1-100 | K. Nasmyth |
| K700 | MATα ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3 can1-100 | K. Nasmyth |
| YFL448/1a | K700 dot1::kanMX6 tel1::HIS3 | This work |
| YFL438 | K699 dot1::kanMX6 mec1-1 sml1 | This work |
| YFL499/3d | K699 dot1::kanMX6 chk1::kanMX6 | This work |
| YFL224 | K699 bre1::kanMX6 | M. Giannattasio |
| YFL236 | K699 set2::kanMX6 | This work |
| YMG203 | K699 bre1::kanMX6 set2::kanMX6 | This work |
| UCC1111 | MATα ade2::his3Δ200 leu2Δ0 lys2Δ0 met15Δ trp1Δ63 ura3Δ0 adh4::URA3-TEL (VII-L) hhf2-hht2::MET15 hhf1-hht1::LEU2(pRS412-ADE2 CEN ARS-HHF2-HHT2) | D. E. Gottschling |
| YFL287 | UCC1111(pMP3) | This work |
| YFL288 | UCC1111(pFL17.5 [H4-K20R]) | This work |
| YFL290 | UCC1111(pFL19.5 [H4-K59R]) | This work |
| YFL292 | UCC1111 dot1::kanMX6(pMP3) | This work |
| YFL294 | UCC1111 dot1::kanMX6(pFL17.5 [H4-K20R]) | This work |
| YFL296 | UCC1111 dot1::kanMX6(pFL19.5 [H4-K59R]) | This work |
| YFP20 | K699 dpb11-1 | This work |
| YFL234 | K699 dot1::kanMX6 | M. Giannattasio |
| YMAG6 | K699 dot1::kanMX6 dpb11-1 | This work |
| YMIC4F6 | K699 mec3::TRP1 rad9::URA3 | This work |
| YLL683.8/3B | K699 ddc2::DDC2-3HA:URA3 | M. P. Longhese |
| YFP24/6b | K699 dpb11-1 ddc2::DDC2-3HA:URA3 | This work |
| YFL403/10b | K699 dot1::kanMX6 ddc2::DDC2-3HA:URA3 | F. Lazzaro |
| YFL687/2b | K699 dot1::kanMX6 dpb11-1 ddc2::DDC2-3HA:URA3 | This work |
| YFL211/3a | K699 RAD9-13myc:TRP1 ddc1::DDC1-HA:LEU2 | This work |
| YMAG48/5b | K700 dpb11-1 RAD9-13myc:TRP1 ddc1::DDC1-HA:LEU2 | This work |
| YMAG34/4a | K699 dot1::kanMX6 RAD9-13myc:TRP1 ddc1::DDC1-HA:LEU2 | This work |
| YMAG52/3d | K699 dot1::kanMX6 dpb11-1 RAD9-13myc:TRP1 ddc1::DDC1-HA:LEU2 | This work |
| YMIC4E8 | K699 rad9::URA3 | F. Lazzaro |
| YMAG149/7B | K699 hta1-htb1::LEU2 hta2-htb2::TRP1(pSAB6 [HTA1-HTB1]) | This work |
| YMAG168 | K699 hta1-htb1::LEU2 hta2-htb2::TRP1(pJD151 [hta1-S129A-HTB1]) | This work |
| YMAG150/4A | K699 dot1::kanMX6 hta1-htb1::LEU2 hta2-htb2::TRP1(pSAB6 [HTA1-HTB1]) | This work |
| YMAG170 | K699 dot1::kanMX6 hta1-htb1::LEU2 hta2-htb2::TRP1(pJD151 [hta1-S129A-HTB1]) | This work |
| YLDN25 | K699 ddc1::kanMX4(pML89) | This work |
| YLDN17 | K699 ddc1::kanMX4(pLD12) | This work |
| YLDN23 | K699 ddc1::kanMX4(pLD26) | This work |
| YLDN24 | K699 ddc1::kanMX4(pLD31) | This work |
| YFP27 | K699 ddc1::kanMX4 dot1::HIS3(pML89) | This work |
| YFP28 | K699 ddc1::kanMX4 dot1::HIS3(pLD12) | This work |
| YFP29 | K699 ddc1::kanMX4 dot1::HIS3(pLD26) | This work |
| YFP30 | K699 ddc1::kanMX4 dot1::HIS3(pLD31) | This work |
| YLDN9 | K699 ddc1::kanMX4(pLD9) | This work |
| YFP37 | K699 ddc1::kanMX4 dot1::HIS3(pLD9) | This work |
| YFP148 | K699 ddc1::kanMX6(pFP9) | This work |
| YFP149 | K699 ddc1::kanMX6 dot1::HIS3(pFP9) | This work |
| YFP38 | K699 dpb11::DPB11-13myc:HIS3 | This work |
| YFP48/3a | K699 dpb11::DPB11-13myc:HIS3 mec1-1 sml1-1 | This work |
| YFP49/1d | K699 dpb11::DPB11-13myc:HIS3 rad53::kanMX6 sml1::HIS3 | This work |
| YFP55/6c | K699 ddc1::kanMX6 dpb11::DPB11-13myc:HIS3 | This work |
| YFP56 | K699 ddc1::kanMX6 dpb11::DPB11-13myc:HIS3(pML89) | This work |
| YFP57 | K699 ddc1::kanMX6 dpb11::DPB11-13myc:HIS3(pLD9) | This work |
| YFP63 | K699 ddc1::kanMX6(pML89) | This work |
| YFP64 | K699 ddc1::kanMX6(pLD9) | This work |
| YFP65 | K699 ddc1::kanMX6 dpb11-1(pML89) | This work |
| YFP66 | K699 ddc1::kanMX6 dpb11-1(pLD9) | This work |
| YFP 152 | K699 ddc1::kanMX6 [Ycplac111) | This work |
| YFP 142 | K699 dot1::HIS3 dpb11-1 ddc1::kanMX6(pML89) | This work |
| YFP 144 | K699 dot1::HIS3 dpb11-1 ddc1::kanMX6(pLD9) | This work |
| YFP50 | EGY48(pSH18.34/pFP1/pFP2) | This work |
| YFP52 | EGY48(pSH18.34/pFP1/pFP4) | This work |
| YFP113 | K699 mec1-1 sml1(pSH18.34/pFP1/pFP2) | This work |
| YFP114 | K699 mec1-1 sml1(pSH18.34/pFP1/pFP4) | This work |
| YFP86 | EGY48(pSH18.34/pJG4-5/pFP2) | This work |
| YFP54 | EGY48(pSH18.34/pFP1/pEG202) | This work |
| YFP153 | EGY48(pSH18.34/pFP1/pFP10) | This work |
| YMAG78/4b | Y1812 ddc2HA-URA3 | This work |
| YMAG82/15a | YJT70 ddc2HA-URA3 | This work |