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. 2008 Jun 2;28(15):4772–4781. doi: 10.1128/MCB.00323-08

FIG. 7.

FIG. 7.

PRC2 components and Snail1 interact in vivo. (A to C) RWP-1 cells (A) and HT-29 M6 (B and C) stably transfected with Snai1-HA were lysed and immunoprecipitated (IP) with antibodies anti-Suz12 (A and B) or anti-HA (C). The immunocomplex was analyzed by Western blotting (WB), using antibodies against HA (A and B), Suz12 (A and B), or Ezh2 (C). IgG, immunoglobulin G. (D) SW-620 cells were lysed and immunoprecipitated with a monoclonal antibody specific for Snail1. The presence of Ezh2 in the immunoprecipitated Snail1 was analyzed by Western blotting (D). (E) RWP-1 cells stably transfected with Snai1-HA or with the Snai1-P2A mutant were immunoprecipitated with an antibody against Suz12. The presence of Snail1-HA and the Snail1-P2A-HA mutant was analyzed by Western blotting. RWP-1 cells stably transfected with pcDNA-3 were used as a control (Cont).