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. 2008 May 27;28(15):4819–4828. doi: 10.1128/MCB.01969-07

FIG. 7.

FIG. 7.

HDAC activity is immunoprecipitated by NFX1-91. (A) Western analysis of immunoprecipitated proteins used in the HDAC assay. (B) HADC assay. FLAG-N91, FLAG-NFX1-123 (FLAG-N123), and FLAG-HDAC2 were overexpressed in 293T cells. Two hours prior to harvesting cells, 10 μM MG-132 was added to the culture. Whole-cell lysates (12.5 mg) were immunoprecipitated with rabbit anti-FLAG antibody. FLAG-HDAC2 was used as a positive control and CMV-based empty vector as a negative control. For endogenous N91, 293T cell lysate (12.5 mg) was immunoprecipitated with rabbit anti-N91 antibody or pre-I serum. HDAC activity was assayed at different time points during incubation.