Table II.
Differentially expressed proteins in A53T α-synuclein flies relative to controls
| Gene name or IDa | FlyBase IDa | Observed ratio ± S.D. (PD-like/control)
|
Total no. peptides | |
|---|---|---|---|---|
| Forward labelingb | Reverse labelingb | |||
| Fat body protein 1 (Fbp1) | FBgn0000639 | 0.41 ± 0.12 (3) | 0.33 ± 0.08 (3) | 4 |
| chaoptic (chp) | FBgn0000313 | 0.41 ± 0.07 (8) | 0.42 ± 0.09 (8) | 9 |
| karst (kst) | FBgn0004167 | 0.51 ± 0.15 (2) | 0.63 (1) | 3 |
| Odorant-binding protein 99b (Obp99b) | FBgn0039685 | 0.53 ± 0.02 (2) | 0.57 ± 0.07 (2) | 2 |
| Cytochrome P450 reductase (Cpr) | FBgn0015623 | 0.60 ± 0.01 (2) | 0.67 (1) | 2 |
| Retinin | FBgn0040074 | 0.61 ± 0.03 (2) | 0.54 (1) | 2 |
| Calnexin 99A (Cnx99A) | FBgn0015622 | 0.61 ± 0.30 (2) | 0.66 (1) | 2 |
| Retinal degeneration A (rdgA) | FBgn0003217 | 0.64 ± 0.03 (2) | 0.60 (1) | 2 |
| Rtnl1 | FBgn0053113 | 0.65 ± 0.08 (4) | 0.64 ± 0.28 (3) | 4 |
| Muscle LIM protein at 60A (Mlp60A) | FBgn0011643 | 1.53 ± 0.11 (2) | 1.72 ± 0.08 (2) | 2 |
| Glycerol-3-phosphate dehydrogenase (Gpdh) | FBgn0001128 | 1.56 ± 0.25 (3) | 1.70 ± 0.26 (3) | 3 |
| Paramyosin (Prm) | FBgn0003149 | 1.57 ± 0.26 (21) | 1.60 ± 0.38 (16) | 23 |
| Superoxide dismutase 2 (manganese) (Sod2) | FBgn0010213 | 1.60 ± 0.26 (2) | 1.84 ± 0.23 (2) | 2 |
| Ribosomal protein L6 (RpL6) | FBgn0039857 | 1.61 ± 0.92 (2) | 1.63 ± 0.76 (2) | 2 |
| Failed axon connections (fax) | FBgn0014163 | 1.66 ± 0.89 (2) | 1.84 ± 1.57 (3) | 3 |
| upheld (up) | FBgn0004169 | 1.82 ± 0.22 (6) | 1.63 ± 0.21 (7) | 9 |
| Heat shock protein cognate 3 (Hsc70-3) | FBgn0001218 | 1.91 ± 1.60 (5) | 2.84 ± 2.72 (3) | 5 |
| Ribosomal protein L14 (RpL14) | FBgn0017579 | 1.94 ± 1.18 (2) | 1.87 ± 1.15 (2) | 3 |
| ATP synthase-β (ATPsyn-β) | FBgn0010217 | 2.30 ± 2.56 (9) | 1.72 ± 1.28 (14) | 15 |
| CG15006 | FBgn0035510 | 2.45 ± 0.05 (2) | 4.42 (1) | 3 |
| CG32029 | FBgn0052029 | 2.52 ± 2.06 (2) | 1.72 ± 0.79 (3) | 3 |
| Ribosomal protein S8 (RpS8) | FBgn0039713 | 3.04 ± 3.26 (3) | 2.64 ± 2.38 (3) | 3 |
| Ribosomal protein L23A (RpL23A) | FBgn0026372 | 3.56 ± 0.52 (2) | 4.21 ± 0.56 (2) | 2 |
| CG7675 | FBgn0038610 | 4.52 (1) | 2.01 ± 0.09 (2) | 2 |
a
Gene name or IDs and FlyBase IDs were obtained from the FlyBase database. Gene symbols are denoted in parentheses to facilitate understanding of Figs. 7 and 8.
b
The numbers in parentheses indicate the total number of peptides used for protein quantification. The observed ratio ± S.D. indicates the average ratio and standard deviation of multiple peptides detected for a protein. Detailed information on peptide identification is in supplemental Table SI.