Fig. 5.

Conformational immunoblots for the selected proteins (Eno1, hnRNP K, and Ly-GDI) that were identified as differentially expressed by 2D DIGE. STZ-induced diabetic rat bladders at two time points was compared with age-matched control rats to identify changes in protein abundances. 50 μg of total protein was loaded in each lane, and the resulting blots were probed with anti-enolase, anti-hnRNP K, and anti-Ly-GDI. Relative -fold changes for these proteins in STZ-induced diabetic rats compared with the age-matched controls are given in Table III. C_1W, 1-week control; D_1W, 1-week diabetes; C_2M, 2-month control; and D_2M, 2-month diabetes.