Skip to main content
. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Kidney Int. 2007 Mar 28;72(2):157–165. doi: 10.1038/sj.ki.5002229

Figure 3. cAMP stimulates cell proliferation in PKD1 cystic but not normal cell lines.

Figure 3

(A) Several agents which increase cellular cAMP concentrations such as dbcAMP (1 mM), forskolin (100 mM) and IBMX (0.5 mM) stimulated an increase in proliferation in the cystic line, SKI-001 but not the normal line, UCL 93/3. The graphs show the data from representative experiments performed in triplicate. (B) The cAMP analogue dbcAMP (1mM) stimulated cell proliferation in 3 PKD1 cystic lines (OX938 C8, OX161 and SKI-001) but not in 3 normal lines (CL11, RFH, UCL93). Of note, the effect of IGF-1 (50 ng/ml) was additive to the cAMP response in all 3 cystic lines. (C) The relative abundance of cell surface IGF-1 receptors between the cystic and normal lines was examined by surface biotinylation of the β subunit of the IGF-1 receptor. There was no difference in total (lysate) or surface (avidin IP) levels of IGF-1 receptors between the two cell lines. The ER resident protein calnexin served as a control for surface biotinylation and for protein loading.