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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Kidney Int. 2007 Mar 28;72(2):157–165. doi: 10.1038/sj.ki.5002229

Figure 5. Knock down of ras isoforms blocked the effect of IGF-1 and cAMP on cystic cell proliferation.

Figure 5

(A) All three major ras isoforms (N-, H-, Ki-) could be detected in normal and cystic cells by RT-PCR (not shown). N-ras was the major isoform expressed by Q-PCR. The specificity for each individual siRNA to knockdown each ras isoform in SKI-001 cells was examined at 48h after transfection by semi-quantitative PCR in the linear range of the curve (21-23 cycles) with a control (actin) reaction. As shown on the gel, the effect was specific for the isoform. The major upper band is actin. (B) Knock down of either N-, H- or Ki-ras was sufficient to block the proliferative effect of either individual (not shown) or combined IGF-1/dbcAMP stimulation. The graph shows data from three experiments performed in triplicate. Scrambled siRNA was used as a negative control.