Figure 4.

Rates of disulfide bond formation in the mutant T242C/S338C in the dark and after light activation. The proteins were eluted from the Ab 1D4-Sepharose™ column in buffer F (Materials and Methods). The samples were maintained throughout at 4°C. After increasing the pH to 7.5, the decrease in sulfhydryl groups was monitored both in the dark and after illumination. The Meta II half-life of T242C/S338C at 4°C was ≈69 min.