Table 1.
Characterization of cysteine substitution mutants of rhodopsin
| Mutants | λmax (nm) | A280:A500 | t1/2 of retinal release (min) |
|---|---|---|---|
| A. Single cysteine mutants | |||
| wt | 500 | 1.6 | 12.8 |
| T335C | 498 | 1.8 | 12.4 |
| T336C | 498 | 1.7 | 11.6 |
| V337C | 498 | 1.7 | 11.7 |
| S338C | 498 | 1.7 | 12.0 |
| K339C | 498 | 1.8 | 11.7 |
| T340C | 498 | 1.7 | 11.4 |
| B. Double cysteine mutants | |||
| S240C/S338C | 499 | 1.6 | 13.9 |
| A241C/S338C | 499 | 1.6 | 8.9 |
| T242C/S338C | 499 | 1.6 | 10.0 |
| T243C/S338C | 499 | 1.6 | 11.4 |
| Q244C/S338C | 499 | 1.6 | 10.7 |
| K245C/S338C | 499 | 1.6 | 8.2 |
| A246C/S338C | 499 | 1.6 | 12.4 |
| E247C/S338C | 499 | 1.6 | 15.8 |
| K248C/S338C | 499 | 1.6 | 9.5 |
| E249C/S338C | 499 | 1.6 | 16.5 |
| V250C/S338C | 499 | 1.5 | 14.7 |
| H65C/S338C | 500 | 1.6 | 10.2 |
| C140/S338C | 499 | 1.7 | 10.7 |