Figure 4.

Overview of silencer and EB sites and their genomic features. (A) Summary of all transfection results organized by conserved and nonconserved categories. Dark gray boxes indicate strong phenotypes for silencing or EB, producing expression levels at 1× or below. (Light gray boxes) Modest silencing or EB phenotypes (at least twofold below the enhancer-control); (white boxes) no decrease in expression or a value less than twofold below the enhancer. Columns below the transfection results indicate characteristics of each element, including activity levels, reversibility, motif content, and genomic features. (B) Analysis of genomic attributes in strong silencers and EBs. The data are graphed to show the percent of NREs and non-NREs carrying each feature.