Figure 1.

Experimental evidence for S. cerevisiae orphan ORFs. (A) Percentages indicate proportions of orphan ORFs detected at least in one of 13 functional genomics and proteomics data sets (Table 1). Note that ORFs rejected by all three comparative genomic studies analyzed here (Brachat et al. 2003; Cliften et al. 2003; Kellis et al. 2003) show similar percentages. (B) Supporting experimental evidence for each of 648 ORFs observed as orphan by three comparative genomic studies (Brachat et al. 2003; Cliften et al. 2003; Kellis et al. 2003). Complete lists of ORFs and supporting experimental evidence are in Supplemental Table 2. Columns are ordered from the ORF with most evidence (ORF X₁; left) to the one with the least evidence (ORF X₆₄₈; right). Data sets were grouped together by type of experimental approach, transcriptional on top and translational at the bottom. In total, there are 477 orphan ORFs with transcriptional evidence, 180 with translational evidence, and 145 with both transcriptional and translational evidence.