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. 2008 Aug;22(8):2723–2733. doi: 10.1096/fj.08-106997

Figure 2.

Figure 2.

NADPH oxidase and ROS are involved in DEP up-regulation of P-glycoprotein. A) Western blot showing expression of NADPH oxidase subunit gp91PHOX in microglia and brain capillary membranes. B) Exposing brain capillaries to DEPs for 6 h increased gp91PHOX expression in membranes. C) DEP exposure of brain capillaries for 30 min increased CM-DCF fluorescence, indicating increased ROS levels. ROS was measured using CM-H2DCFDA; values are means ± se (au; n=3) for capillaries from a single preparation (pooled tissue from 10 rats). D) NADPH oxidase inhibition with DPI abolished the DEP-induced increase of ROS (measured as CM-DCF fluorescence) in isolated brain capillaries. Data are percentages of control levels. E) Inhibition of NADPH oxidase with DPI blocked DEP up-regulation of P-glycoprotein expression (Western blot) and transport activity (specific luminal NBD-CSA fluorescence). F) Inhibition of NADPH oxidase with apocynin also blocked the DEP effect on P-glycoprotein. Specific luminal NBD-CSA fluorescence values are means ± se for 10 capillaries from a single preparation (pooled tissue from 10 rats); scale 0–255 au. ***P < 0.001.