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. 2008 Aug;22(8):2723–2733. doi: 10.1096/fj.08-106997

Figure 3.

Figure 3.

ROS scavengers prevent P-glycoprotein up-regulation. A) The ROS scavenger SOD abolishes DEP up-regulation of P-glycoprotein expression (Western blot) and transport activity (specific luminal NBD-CSA fluorescence). B) Catalase, another ROS scavenger, also abolishes the effect of DEPs on P-glycoprotein. C) The antioxidant N-acetyl-l-cysteine (NAC) blocks DEP up-regulation of P-glycoprotein. D) Western blot showing expression of the microglia marker Iba-1. Iba-1 is expressed in microglia (positive control) and total brain, but not in brain capillaries. Specific luminal NBD-CSA fluorescence values are means ± se for 10 capillaries from a single preparation (pooled tissue from 10 rats); scale 0–255 au. ***P < 0.001.