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. 2008 Aug;22(8):3010–3023. doi: 10.1096/fj.07-100966

Figure 1.

Figure 1.

Ang1 mRNA levels change during cardiac remodeling. A) RT-PCR analysis of mRNA transcripts in mouse LV, RV, and AT. From neonate to adult, ang1 and VEGF188 mRNA expressions increase, whereas VEGF120 mRNA decreases. B) Western blotting shows corresponding increases in ang1 protein levels in mouse LV with age. *P ≤ 0.05 vs. 2 days. C) Blots show ang1 mRNA levels in rat CMs: trace levels in neonatal rats; abundant in adults. Studies done in duplicate; n = 4/group (A–C). D–F) Real-time PCR analysis of ang1 mRNA in mice: levels in LV (D), RV (E), and AT (F) increase with age. Insets (dashed boxes) magnify days –1 to 21. Studies done in triplicate; n = 5/group. G, H) Analysis of mice treated with PE or saline (control): PE increases LV/tibia ratios (G); real-time PCR shows LV ang1 mRNA levels decrease with PE-induced hypertrophy.