Figure 7.

Effects of Gα12Q229L mutants on JNK and Bcl-2. COS-7 cells were transfected with either pcDNA3.1-Myc-His₆ or Gα12Q229L-Myc constructs as indicated. A) The presence and phosphorylation levels of JNK (p46) and Bcl-2 (phosphorylated at Ser-70), as well as Myc-tagged Gα12 and COX (as a mitochondrial marker) were detected by Western blotting using respective antibodies (c, control). The diagrams show quantification of the ECL data shown in A: effects of Gα12Q229L-Myc mutants on phosphorylation state of JNK (p46) (B), or Bcl-2 (C), or on the cellular levels of Bcl-2 (D). Data are means of 3 replicates; error bars indicate sd. The experiment was repeated twice (3 replicates in each experiment) with similar results.