Table 2.

Binding (fmoles) of [¹²⁵I]A-85380 in the presence of 100 nM αCtxMII (to α4β2* nAChRs)

		Adults		Periadolescents		Treatment effects		Age effects
	Brain Region	Saline	Nicotine	Saline	Nicotine	% chg from saline		% chg from adult
		Mean+/−sem				Adult	Periadol.	Saline	Nicotine
1	Nu. accumbens, core	3.10±0.35	5.16±0.43	5.48±0.62	7.05±0.51	66.4^**	28.5^*	76.8^**	36.5^*
2	Nu. accumbens, shell	2.72±0.33	5.08±0.46	5.22±0.64	7.16±0.49	86.7^**	37.2^*	91.8^**	40.9^*
3	Endopiriform nu.	3.91±0.33	7.48±0.30	5.92±0.68	8.32±0.22	91.1^***	40.4^**	51.4^**	11.3
4	Striatum, rostral	3.94±0.39	5.97±0.41	6.09±0.59	7.87±0.30	51.4^*	29.2^*	54.8^**	31.8^*
5	Striatum, caudal	3.39±0.35	5.36±0.50	5.40±0.66	7.13±0.48	58.3^**	32.1^**	59.2^**	32.9
6	Lateral septum	3.65±0.37	5.20±0.56	6.00±0.63	7.25±0.47	42.6^*	20.8	64.6^**	39.5^*
7	Medial habenula	8.14±0.26	8.49±0.15	8.70±0.04	8.57±0.13	4.3	−1.5	6.9	0.9
8	Fasiculus retroflexus	6.02±0.36	6.96±0.32	7.66±0.35	8.19±0.25	15.6	6.9	27.2^**	17.7^*
9	Posterior hypothalamus	1.29±0.18	4.38±0.65	2.06±0.32	5.41±0.71	239^**	162^***	59.4	23.3
10	Ventral posterior thal. nu.	6.12±0.44	8.21±0.32	7.54±0.41	8.50±0.21	34.2^**	12.8	23.2^*	3.5
11	Posterior thal. nu. group	6.91±0.41	8.46±0.24	8.08±0.27	8.63±0.13	22.4^**	6.8	16.9^**	2.0
12	Lateral post. thal. nu.	7.14±0.37	8.32±0.27	8.14±0.26	8.59±0.14	16.6^*	5.6	14.1^*	3.3
13	Internal capsule	1.52±0.17	2.32±0.33	4.12±0.71	4.82±0.66	52.6	16.8	171^*	107
14	Optic tract	1.92±0.20	2.00±0.32	4.58±0.87	5.28±0.63	4.6	15.2	139^**	163^*
15	Optic chiasm	5.88±0.45	6.98±0.39	7.43±0.44	8.16±0.28	18.7	9.9	26.4^*	16.9^**
16	Superior collic., super. grey	6.11±0.54	7.29±0.30	7.56±0.42	8.09±0.32	19.5	7.0	23.7	10.8^*
17	Dorsal lateral geniculate	7.91±0.27	8.78±0.04	8.47±0.15	8.65±0.12	11.0	2.2	7.1	−1.4
18	Ventral lateral geniculate	5.67±0.60	6.32±0.30	6.70±0.59	7.71±0.46	11.5	15.1	18.2^*	22.2
19	Medial geniculate	6.24±0.45	8.07±0.22	7.71±0.39	8.39±0.23	29.3^**	8.9	23.4^**	4.0
20	Zona incerta	2.44±0.29	5.84±0.67	4.63±0.66	6.96±0.55	140^**	50.2^*	90.0^**	19.1
21	Tectal nuclei	5.44±0.56	6.02±0.30	6.72±0.52	7.46±0.47	10.7^*	11.1	23.6^**	24.1
22	Substantia nigra, P.C.	6.07±0.75	7.44±0.55	8.06±0.26	8.31±0.28	22.6	3.0	32.8	11.6
23	Ventral tegmental area	4.88±0.60	6.05±0.56	6.89±0.52	7.99±0.32	24.1^*	16.1	41.3^*	32.2
24	Dentate gyrus, hippocampus	2.35±0.31	5.00±0.61	4.27±0.62	7.09±0.44	112^**	66.0^*	81.5^*	41.8
25	CA1, hippocampus	1.21±0.13	3.29±0.48	2.29±0.37	5.06±0.68	171^**	120^**	89.1^*	53.7
26	Subiculum	3.61±0.42	4.93±0.29	5.79±0.62	6.52±0.56	36.5^*	12.7	60.2^*	32.3
27	Postsubiculum	7.23±0.37	8.25±0.16	8.37±0.18	8.60±0.14	14.1^**	2.8	15.7^**	4.3
28	Pontine nu.	2.40±0.47	3.98±0.44	4.11±0.77	5.99±0.67	66.1	45.6	71.7^*	50.5
29	Entorhinal cortex	3.01±0.37	6.50±0.24	5.15±0.70	7.33±0.50	116^**	42.3^*	71.1^**	12.8
30	Frontal cortex, L4	4.08±0.40	6.59±0.50	6.04±0.59	7.83±0.38	61.6^**	29.7^*	48.1^**	18.9
31	Frontal cortex, L5	3.10±0.32	6.70±0.38	5.20±0.66	7.58±0.44	116^***	45.6^*	67.9^**	13.0
32	Cingulate cortex, outer	5.48±0.40	7.09±0.36	7.35±0.46	8.19±0.28	29.3^**	11.4	34.3^**	15.6
33	Cingulate cortex, inner	4.89±0.43	7.13±0.38	6.91±0.54	8.45±0.16	45.9^**	22.2	41.4^**	18.5
34	Retrosplenial cortex, L1–2	6.79±0.41	8.04±0.18	8.07±0.33	8.57±0.14	18.4^**	6.2	18.8^**	6.6
35	Retrosplenial cortex, L3–4	6.36±0.44	7.93±0.18	7.93±0.37	8.44±0.19	24.7^**	6.5	24.6^**	6.5
36	Visual cortex, L4	4.95±0.50	7.54±0.31	6.70±0.61	8.11±0.32	52.4^**	21.0	35.5^*	7.6
37	Visual cortex, L5	3.42±0.44	6.44±0.53	5.60±0.68	7.72±0.42	88.0^***	37.9^*	63.4^**	19.8
38	Visual cortex, L6	4.47±0.44	6.73±0.32	6.53±0.63	7.87±0.37	50.7^**	20.5	46.1^**	16.9

Means determined from 5–8 replicates. Means from all four groups compared by 2-way ANOVA with Holm-Sidak post-test

p<0.05

^**

p<0.01

^***

p<0.001.