Figure 3.

Antigenic reactivity of mutated antibodies in comparison to their unmutated counterparts. The reactivity of three mutated antibodies and their germline counterparts to different antigens was tested by ELISA at 5 μg/mL and three subsequent 1:4 dilutions. Clone 53 from MC Nussenzweig’s laboratory was used as a negative control (dotted lines). Mutated antibodies and unmutated counterparts are shown in the same color (A9: black; B5: red and G3: green). Triangles and squares represent mutated antibodies and their unmutated counterparts, respectively.