Table 1.

Effect of stimulation and HKa on expression of uPARs

	pro-uPA	uPA	uPA/PAI-1	VEGF	bFGF	PMA
Cell surface uPAR concentrations
Unstimulated cells	9.07±0.06	9.86±0.14	9.06±0.03	8.88±0.04	7.24±0.06	7.58±0.03
Stimulated cells	9.54±0.09	6.01±0.06	6.37±0.05	5.12±0.04	5.18±0.01	8.3±0.01
Stimulated cells + HKa	8.88±0.22	8.15±0.14	8.66±0.08	8.58±0.03	8.06±0.18	10.41±0.23
Probability values for differences
Unstimulated cells vs. stimulated cells	0.011	<0.001	<0.001	<0.001	<0.001	<0.001
Stimulated cells vs. stimulated cells + HKa	0.050	<0.001	<0.001	<0.001	<0.001	<0.001
Unstimulated cells vs. stimulated cells + HKa	0.229	0.001	0.008	0.002	0.012	<0.001

Values for cell surface urokinase plasminogen activator (uPA) receptor (uPAR) concentrations are means ± SE (in arbitrary fluorescence units). Cell surface uPARs were measured by immunocytofluorimetric analysis as described in materials and methods. PAI-1, plasminogen activator inhibitor-1; bFGF, basic FGF. For cell surface uPAR concentrations, unstimulated cells represent cells without treatment as the control, stimulated cells represent cells treated with agonists, and stimulated cells + two-chain high-molecular-weight kininogen (HKa) represent cells preincubated with HKa (300 nM) before the addition of corresponding agonists. Immunocytofluorimetric data were collected from 3 independent experiments and quantified by FlowJo 7.2.2 software. For probability values for differences, comparisons of one group to the other group and statistical analysis were performed as described in materials and methods. Results were considered significant when P < 0.05.