Fig. 2.

Rho-GTPase activity is correlated with thrombin induced ATP release. A: photographs were taken of serum-starved 1321N1 cells maintained treated for 3 h with 1:50 dilution of ToxB or treated for 6 h with 2 μg/ml C3. B: G-LISA was performed as described in methods. Data represent means ± SE of 3 independent experiments each performed in duplicate for control and C3 groups and two independent experiments each performed in duplicate for the ToxB groups. *P < 0.05 vs. thrombin-treated control. #P = 0.06 vs. thrombin-treated control. C: 1321N1 cells were treated with 300 μM βγ-meATP for 15 min. A sample of the reaction media was taken for a baseline [ATP] measurement (openr bars) and then cells were treated with 2 U/ml thrombin for 15 min before removing a second sample of the reaction media (solid bars). Extracellular ATP concentration was measured via an off-line luciferin/luciferase assay as described in methods. Data represent means ± SE of 4 independent experiments each performed in triplicate. ***P < 0.001 vs. thrombin-treated control.