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. 2008 May 21;295(1):C231–C241. doi: 10.1152/ajpcell.00175.2008

Fig. 4.

Fig. 4.

Effects of ToxB and BAPTA-loading on ATP release from 1321N1 astrocytes in response to lysophosphatidic acid (LPA) and carbachol. Changes in stimulated extracellular [ATP] were recorded in control (▪) cells versus ToxB-pretreated (▴), or BAPTA-pretreated (▴) cells as described under methods. A: on-line ATP measurements were made every 2 min after addition of 300 μM βγ-meATP concurrent with 10 μM LPA. *P < 0.05 vs. LPA-treated control. The differences between the control and the treated groups were significant (P < 0.05) from t = 2-min through t = 12-min time points. B: on-line ATP measurements were made every 2 min after addition of 300 μM βγ-meATP concurrent with 100 μM carbachol. *P < 0.05 vs. carbachol-treated control. The differences between the control and the BAPTA-treated groups was significant (P < 0.05) from the t = 2-min through the t = 12-min time points. The differences between the control and the ToxB-treated groups were significant (P < 0.05) at the t = 10 min and t = 12-min time points. Data for both panels represent means ± SE of 3 independent experiments each performed in triplicate.