Fig. 6.
Aspartate aminotransferase inhibitors do not affect the ability of DMOG-treated cultures to maintain mitochondrial membrane potential (ΔΨmito) using fumarate respiration in metabolic inhibition (MI) conditions. Cultures were treated as described in Fig. 5 legend with cyanide and 2-DG for 2 h in the presence of 1 mM aminooxyacetate or 1 mM l-vinyl glycine. JC-1 was loaded during the final 15 min of MI. ΔΨmito is expressed as ratio of 590-nm to 530-nm fluorescence as quantified with a fluorescent plate reader after JC-1 staining (n = 6) under respective conditions. The mitochondrial uncoupler carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone was used a positive control (not shown). Values are means ± SE (n = 8). *Significantly different from corresponding control (P < 0.05, by t-test).