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. 2008 May 21;295(1):C92–C99. doi: 10.1152/ajpcell.00023.2008

Fig. 3.

Fig. 3.

Effect of PIP2 on single TRPA1 channels. A: an inside-out patch was formed with 5 mM PPPi and 20 μM AITC in the pipette. Patches showing a low level of channel activity were used. Low concentrations of PIP2 were then applied to the bath solution. Channel openings at time points a through f are shown at expanded scale in B. C: amplitude histograms were obtained from channel recordings at different [PIP2], and typical histograms are shown. D: duration histograms were obtained from patches showing mainly one level of opening. The duration histograms were fitted with two exponential probability function. E: graph shows the effect of PIP2 on single channel conductance determined at −40 mV and +40 mV. Each bar is the mean ± SD of 3 determinations. No significant differences were present at the same membrane potentials (P > 0.05). F: graph shows the effect of PIP2 on two mean open times (τo1 and τo2) at each [PIP2]. PIP2 produced a progressive reduction in τo2. The percent values shown in the open bars indicate the relative contribution of the longer-open state to the total current.